We are conducting basic research to clarify the response characteristics of microalgae to the light environment at the molecular level, and research to create new species of microalgae. Microalgaes are an interesting group of organisms that have undergone various evolutions on this earth for more than 2 billion years, and many microalgaes accumulate lipids (oils). Attention is focused on the overwhelmingly high lipid production efficiency per unit area of microalgae, which means that it is expected to be used as a raw material for future biofuels. In our laboratory, we are actively conducting joint research with other universities and companies, along with graduate research students, Graduate School, postdoctoral researchers, and international students from overseas.
Faculty name/Affiliation | Tomoko Shinomura / Department of Biosciences Faculty of Faculty of Science and Engineering and Engineering |
---|---|
Specialized Fields | Plant physiology, global environmental biology |
Research theme | Basic research on microalgae and applied research on biofuels |
Research keywords | Energy, cell cycle, light, Euglena, Euglena, plants, ecosystems, plankton, microorganisms, evolution, Pediastrum, green algae, molecular level |
Faculty introduction URL | https://www3.med.teikyo-u.ac.jp/profile/ja.bf89c22522017c80.html |
Elucidation of molecular mechanism for improving resistance to strong light stress of microalgae
The "strong light stress environment" is a major environmental factor that hinders the efficient cultivation and production of the microalgae Euglena, which is expected to be useful as a raw material for jet fuel, outdoors. Many microalgae have a problem that their growth is inhibited under too strong light such as the direct light of the sun, and the solution is an important research theme. In this study, we focus on carotenoids, which play an important role in intense light stress, clone all enzyme genes of the carotenoid synthesis system in Euglena, and proceed with detailed functional analysis and regulatory mechanism analysis. By doing this, we are aiming to clarify the relationship with strong light stress tolerance and to contribute to the highly efficient production of microalgae biomass.
Elucidation of the light regulation mechanism of the life history of microalgae
"Light" plays an important role in the growth and differentiation of microalgae. Regulation by light can be divided into "control through the drive of the photosynthetic system" and "control through the transmission of optical signals", but research to strictly distinguish between these has not been advanced in microalgae. We examine in detail the asexual reproduction cycle of Futatsunokunshomo isolated and cultured from the forest pond on the grounds of Forest Utsunomiya and Culture, and examine how its life history is regulated by light with a confocal laser. We aim to elucidate the detailed molecular mechanism by microscopic observation and transcriptome analysis.
Joint research with companies on biofuel production technology using microalgae
Through joint research with companies developing biofuels, we are working to connect our cutting-edge research to applications. Future progress is expected in the analysis of environmental responses at the genetic level of microalgae and molecular breeding.
Title | Journal title | Laboratory | Contents |
---|---|---|---|
Diverse functions of carotenoids in light environmental response of Euglena | Oreoscience Vol.23 No.2 (2023) pp.79-86 | Plant Molecular Cytology Laboratory | detail |
Chemical and genetic carotenoid deficiency delays growth in dark-grown Euglena gracilis | Bioscience, Biotechnology, and Biochemistry, 87(5):491-500, | Laboratory of Plant Molecular Cytology / Laboratory of Phytochemistry | detail |
Title | Journal title | Laboratory | Contents |
---|---|---|---|
Temporal Evolution of the Gravitaxis of Euglena gracilis from a Single Cell | Plants 2021, 10, 1411. Https://doi.org/10.3390/plants10071411 | Plant Division Cytology Laboratory | detail |
Regeneration of the Eyespot and Flagellum in Euglena gracilis during Cell Division | Plants 2021, 10, 2004. https://doi.org/10.3390/plants10102004 | Plant Division Cytology Laboratory | detail |
Suppression of the Lycopene Cyclase Gene Causes Downregulation of Ascorbate Peroxidase Activity and Decreased Glutathione Pool Size, Leading to H2O2 Accumulation in Euglena gracilis | Frontiers in Plant Science, 03 December 2021 | https://doi.org/10.3389/fpls.2021.786208 | Plant Division Cytology Laboratory | detail |
Title | Journal title | Laboratory | Contents |
---|---|---|---|
Carotenoid accumulation in the eyespot apparatus required for phototaxis is independent of chloroplast development in Euglena gracilis | Plant Science | Plant Molecular Cytology Laboratory | detail |
Light dependent accumulation of β-carotene enhances photo-acclimation of Euglena gracilis | Journal of Photochemistry & Photobiology, B: Biology | Plant Molecular Cytology Laboratory | detail |
Title | Journal title | Laboratory | Contents |
---|---|---|---|
Phototaxis and photo-shock responses of Euglena gracilis under gravitaxis. | Algal Research, Volume 41, 2019, 101563, Pages 1-11, | Plant Molecular Cytology Laboratory | detail |
Microalgae eye spot structure and function in photomotor response | Journal of Japanese Society of Plant Morphology Plant Morphology, 31 (No.1): 3-9. | Plant Molecular Cytology Laboratory | detail |
Carotenoid in the eyespot apparatus is required for triggering phototaxis in Euglena gracilis. | Plant Journal, 20 October 2019 | Plant Molecular Cytology Laboratory | detail |
Oxygenic Phototrophs Need ζ-Carotene Isomerase (Z-ISO) for Carotene Synthesis: Functional Analysis in Arthrospira and Euglena. | Plant and Cell Physiology, Volume, 61, Issue, (2), 1 February 2020, Pages, 276-282.doi: 10.1093 / pcp / pcz192. | Plant Molecular Cytology Laboratory | detail |
Title | Journal title | Laboratory | Contents |
---|---|---|---|
Carotenoids: New developments pioneered by their diversity and universality | Forefront of Plant Science (BSJ-Review) | Plant Molecular Cytology Laboratory | detail |
Photoresponse through carotenoids of Euglena gracilis and its physiological and ecological functions | Forefront of Plant Science (BSJ-Review) | Plant Molecular Cytology Laboratory | detail |
Physiological role of β-carotene monohydroxylase (CYP97H1) in carotenoid biosynthesis in Euglena gracilis | Plant Science | Plant Molecular Cytology Laboratory | detail |
Low temperature stress alters the expression of phytoene desaturase genes (crtP1 and crtP2) and ζ-carotene desaturase genes (crtQ) of Euglena gracilis and the cellular carotenoid content. | Plant and Cell Physiology | Plant Molecular Cytology Laboratory | detail |
Title | Journal title | Laboratory | Contents |
---|---|---|---|
Suppression of the phytoene synthase gene (EgcrtB) alters carotenoid content and intracellular structure of Euglena gracilis. | BMC Plant Biology | Plant Molecular Cytology Laboratory | detail |
Title | Laboratory | Contents |
---|---|---|
Identification and functional analysis of the geranylgeranyl pyrophosphate synthase gene (crtE) and phytoenesynthase gene (crtB) for carotenoid biosynthesis in Euglena gracilis. | Plant Molecular Cytology Laboratory | detail |
Title | Society name | Laboratory | Contents |
---|---|---|---|
Euglena carotenoids are involved in stress tolerance in a photoprotectant-dependent manner | The 85th Annual Meeting of the Botanical Society of Japan (September 16, 2021 [Sun] -20 [Sun], Tokyo University Gakunan-Ozawa Campus, Online) | Plant Division Cytology Laboratory | detail |
Colonial green alga Pediastrum's new colonial formation is regulated in a reversible manner. | The 85th Annual Meeting of the Botanical Society of Japan (September 16, 2021 [Sun] -20 [Sun], Tokyo University Gakunan-Ozawa Campus, Online) | Plant Division Cytology Laboratory | detail |
Carotenoids are also involved in Euglena cell proliferation in the dark | Euglena Study Group 36th Workshop (October 30, 2021 [May], Teikyo University Utsunomiya Campus, Online) | Plant Division Cytology Laboratory | detail |
Colonial green algae Pediastrum / distant light reversible new colony formation | Euglena Study Group 36th Workshop (October 30, 2021 [May], Teikyo University Utsunomiya Campus, Online) | Plant Division Cytology Laboratory | detail |
Effect of Euglena Carotenoid Synthesis on Ascorbic Acid-Glutathione Cycle | Euglena Study Group 36th Workshop (October 30, 2021 [May], Teikyo University Utsunomiya Campus, Online) | Plant Division Cytology Laboratory | detail |
Title | Society name | Laboratory | Contents |
---|---|---|---|
Functional analysis of β-carotene hydroxylase (CYP97H1) in Euglena | 26th Algae Joint Seminar | Plant Molecular Cytology Laboratory | detail |
Analysis of eye spots and phototaxis in Euglena, which suppresses carotenoid synthesis | The 83rd Annual Meeting of the Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
Molecular biological approach to euglena carotenoid-mediated photoenvironmental response | Euglena Study Group 35th Workshop (Invited Lecture) | Plant Molecular Cytology Laboratory | detail |
Functional analysis of lycopene cyclase involved in Euglena carotenoid synthesis | Euglena Study Group 35th Workshop | Plant Molecular Cytology Laboratory | detail |
Elucidation of the function of eye spot carotenoids in the photomotion of the microalga Euglena Functional analysis of eyespot-localized carotenoids on photomovement in Euglena gracilis | The 42nd Annual Meeting of the Molecular Biology Society of Japan | Plant Molecular Cytology Laboratory | detail |
Title | Society name | Laboratory | Contents |
---|---|---|---|
Static and dynamic: Euglena's photokinetic response to be read by electron microscope and motion analysis | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
Effect of light environment on carotenoid synthesis of microalgae Euglena gracilis | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
Gene Expression profiling and Genetic transformation trials on Pediastrum duplex, a model for photoregulation of life cycle. | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
Effect of blue light irradiation on carotenoid composition and photosynthetic activity of Euglena gracilis | Euglena Study Group | Plant Molecular Cytology Laboratory | detail |
Blocking of carotenoid biosynthesis caused a defect in the normal eyespot formation and resulted ina loss of phototaxis of Euglena gracilis | Japan-Taiwan Plant Biology 2019 | Plant Molecular Cytology Laboratory | detail |
Blue light dependent carotenoid biosynthesis and its effects on photo-acclimation in Euglena gracilis | The 60th Annual Meeting of the Japanese Society of Plant Physiology (Nagoya) | Plant Molecular Cytology Laboratory | detail |
Light-regulation in Pediastrum duplex: Physiological outcomes and Gene expression analysis | The 60th Annual Meeting of the Japanese Society of Plant Physiology (Nagoya) | Plant Molecular Cytology Laboratory | detail |
Title | Society name | Laboratory | Contents |
---|---|---|---|
Light stress alters carotenoid content and intracellular structure of Euglena gracilis | International Symposium on Carotenoids | Plant Molecular Cytology Laboratory | detail |
Analysis of the physiological effects of jasmonic acid on the regulation of Euglena carotenoid synthesis | Euglena Study Group | Plant Molecular Cytology Laboratory | detail |
Effect of light-dark cycle culture on carotenoid synthesis of Euglena gracilis | Euglena Study Group | Plant Molecular Cytology Laboratory | detail |
Light-guided microalgae carotenoid research begins | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
Photoresponse through carotenoids of Euglena gracilis and its physiological and ecological functions | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
Regulation of carotenoid synthesis and accumulation of Euglena gracilis by jasmonates | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
Analysis of carotenoid synthesis of the microalgae Euglena gracilis in light-dark cycle cultures | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
Analysis of light regulated gene expression during cell cycle in Pediastrum duplex. | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
RNA sequencing analysis for differentially expressed genes during light regulation of cell cycle in Pediastrum duplex. | Algae Joint Seminar | Plant Molecular Cytology Laboratory | detail |
Search for OPDA reductase gene in the microalgae Euglena gracilis | Society of Phytochemical Regulation | Plant Molecular Cytology Laboratory | detail |
LOW TEMPERATURE STRESS ALTERS THE CAROTENOID CONTENT AND COMPOSITION OF A UNICELLULAR ALGA, Euglena gracilis | Asia and Oceania Conference on Photobiology | Plant Molecular Cytology Laboratory | detail |
Photoregulation of carotenoid synthesis in Euglena gracilis. | Asia and Oceania Conference on Photobiology | Plant Molecular Cytology Laboratory | detail |
EgcrtB-suppressed Euglena gracilis is imperceptive to the light direction | Utsunomiya University Optobio Symposium 2017 | Plant Molecular Cytology Laboratory | detail |
Photoregulation of carotenoid synthesis in the microalgae Euglena | Utsunomiya University Optobio Symposium 2017 | Plant Molecular Cytology Laboratory | detail |
RNA sequencing analysis of photo-dependent life cycle of Pediastrum duplex | Utsunomiya University Optobio Symposium 2017 | Plant Molecular Cytology Laboratory | detail |
Functional analysis of jasmonic acid in Euglena cells cultured in strong light environment | Utsunomiya University Optobio Symposium 2017 | Plant Molecular Cytology Laboratory | detail |
Isolation and functional analysis of the jasmonic acid synthesis gene OPR in Euglena | Utsunomiya University Optobio Symposium 2017 | Plant Molecular Cytology Laboratory | detail |
Carotenoid synthesis of Euglena gracilis grown under light / dark cycle and monochromatic illumination | International Symposium on Plant Photobiology 2018 | Plant Molecular Cytology Laboratory | detail |
Identification of molecular factors involved in light signal promotion of life cycle in Pediastrum duplex. (Tentative) | International Symposium on Plant Photobiology 2018 | Plant Molecular Cytology Laboratory | detail |
Light in the world of microalgae: A key research to solve the molecular riddles of evolution and the economic importance | Daegu Gyeongbuk Institute of Science and Technology (DGIST) Institute for Basic Science (IBC), Center for Plant Aging Research | Plant Molecular Cytology Laboratory | detail |
Biosynthesis and accumulation of carotenoids in Euglena gracilis in response to external light cuese | Daegu Gyeongbuk Institute of Science and Technology (DGIST) Institute for Basic Science (IBC), Center for Plant Aging Research | Plant Molecular Cytology Laboratory | detail |
Identification and functional analysis of OPDA reductase (OPR) gene of Euglena gracilis | Annual Meeting of the Japanese Society of Plant Physiology | Plant Molecular Cytology Laboratory | detail |
Photo-control of Carotenoid Synthesis in Euglena gracilis | Annual Meeting of the Japanese Society of Plant Physiology | Plant Molecular Cytology Laboratory | detail |
Gene expression profiling: Identification of factors involved in light signal dependent life cycle of Pediastrum duplex. | Annual Meeting of the Japanese Society of Plant Physiology | Plant Molecular Cytology Laboratory | detail |
Title | Society name | Laboratory | Contents |
---|---|---|---|
Strong light stress response of the carotenoid synthesis system of the microalga Euglena (2pE12) | Japanese Society of Plant Physiology | Plant Molecular Cytology Laboratory | detail |
Cell proliferation and carotenoid analysis in the phytoene synthase gene (EgcrtB) of the microalga Euglena (PL-062) | Japanese Society of Plant Physiology | Plant Molecular Cytology Laboratory | detail |
Analysis of photoregulators involved in the asexual cycle of the colonial green alga Pediastrum | Japanese Society of Plant Physiology | Plant Molecular Cytology Laboratory | detail |
Analysis of carotenoid composition and chloroplast structure of the microalgae Euglena gracilis under light stress | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
Regulation of phytoene synthase gene (EgcrtB) expression in the microalga Euglena | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
Search for jasmonic acid synthesis genes in the microalgae Euglena gracilis | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
Analysis of photoregulators involved in the progression of the elementary processes of the asexual reproduction cycle of the colonial green alga Pediastrum duplex | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
Analysis of the physiological function of jasmonic acid in the photostress response of the microalgae Euglena gracilis | Society of Phytochemical Regulation | Plant Molecular Cytology Laboratory | detail |
Analysis of the physiological function of jasmonic acid in the photoenvironmental response of Euglena gracilis | Euglena Study Group | Plant Molecular Cytology Laboratory | detail |
Analysis of the effect of light-dark cycle on gene expression of Euglena gracilis carotenoid synthesis system | Euglena Study Group | Plant Molecular Cytology Laboratory | detail |
Effect of strong light on photosynthetic pigment content of microalgae Euglena | Utsunomiya University Opto-Bio Symposium | Plant Molecular Cytology Laboratory | detail |
Effect of suppression of phytoene synthase gene expression in the microalga Euglena on carotenoid composition | Utsunomiya University Opto-Bio Symposium | Plant Molecular Cytology Laboratory | detail |
Search and expression analysis of jasmonic acid synthesis genes in the microalgae Euglena gracilis | Utsunomiya University Opto-Bio Symposium | Plant Molecular Cytology Laboratory | detail |
Analysis of carotenoid synthesis gene expression in light-dark cycle culture of microalgae Euglena gracilis | Utsunomiya University Opto-Bio Symposium | Plant Molecular Cytology Laboratory | detail |
Analysis of diurnal changes in nitrogen concentration in the medium in outdoor culture of Euglena | Utsunomiya University Opto-Bio Symposium | Plant Molecular Cytology Laboratory | detail |
Photoregulation involved in the progression of the elementary processes of the asexual reproduction cycle of the colonial green alga Pediastrum | Utsunomiya University Opto-Bio Symposium | Plant Molecular Cytology Laboratory | detail |
Effect of addition of jasmonates on euglena chlorophyll content | Utsunomiya University Opto-Bio Symposium | Plant Molecular Cytology Laboratory | detail |
Light-regulation of asexual reproduction in Pediastrum duplex | Japanese Society of Plant Physiology | Plant Molecular Cytology Laboratory | detail |
Effects of jasmonates on the content of photosynthetic pigments in Euglena gracilis | Japanese Society of Plant Physiology | Plant Molecular Cytology Laboratory | detail |
Functional characterization of β-carotene hydroxylase genes in microalga Euglena gracilis | Japanese Society of Plant Physiology | Plant Molecular Cytology Laboratory | detail |
Title | Society name | Laboratory | Contents |
---|---|---|---|
Basic research on biofuel production using microalgae | Plant electron microscope summer seminar | Plant Molecular Cytology Laboratory | detail |
Elucidation of the function of carotenoids in the microalga Euglena under intense light stress. | Society of Phytochemical Regulation | Plant Molecular Cytology Laboratory | detail |
Isolation and functional analysis of Euglena's phytoene synthase gene crtB | Euglena Study Group | Plant Molecular Cytology Laboratory | detail |
Light induction of daughter colonization in the colonial green alga Pediastrum | Optobio Symposium | Plant Molecular Cytology Laboratory | detail |
Effects of light environmental factors on Euglena cells | Optobio Symposium | Plant Molecular Cytology Laboratory | detail |
Strong light stress response of the carotenoid synthesis system in the microalga Euglena | Optobio Symposium | Plant Molecular Cytology Laboratory | detail |
Isolation and functional analysis of Euglena carotene synthesis genes | Annual Meeting of the Japanese Society of Plant Physiology | Plant Molecular Cytology Laboratory | detail |
Expression of phytoene synthase gene in Euglena gracilis and its responses to cold-light stress | International Meeting on Biosynthesis, Functions and Synthetic Biology of Isoprenoids | Plant Molecular Cytology Laboratory | detail |
Temperature and photoenvironmental response of carotenoid synthesis system of microalgae Euglena gracilis | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
The effect of light and temperature on the polynuclearization of the colonial green alga Pediastrum duplex | Botanical Society of Japan | Plant Molecular Cytology Laboratory | detail |
Measurement and functional analysis of endogenous jasmonic acid in the microalgae Euglena gracilis | Society of Phytochemical Regulation | Plant Molecular Cytology Laboratory | detail |
Strong light stress response of carotenoid synthesis system of microalgae Euglena gracilis | Utsunomiya University Optobio Symposium | Plant Molecular Cytology Laboratory | detail |
Effect of suppression of expression of phytoene synthase gene (EgcrtB) in Euglena microalgae on cell proliferation | Utsunomiya University Optobio Symposium | Plant Molecular Cytology Laboratory | detail |
Analysis of photoregulators involved in the asexual cycle of the colonial green alga Pediastrum | Utsunomiya University Optobio Symposium | Plant Molecular Cytology Laboratory | detail |
Effect of light environment during culture on the amount of endogenous jasmonic acid in the microalgae Euglena gracilis | Utsunomiya University Optobio Symposium | Plant Molecular Cytology Laboratory | detail |
Effect of salicylic acid on the growth of the microalgae Euglena | Utsunomiya University Optobio Symposium | Plant Molecular Cytology Laboratory | detail |
Synchronous analysis of new colonial formation in colonial green alga Pediastrum 1 colony | Utsunomiya University Optobio Symposium | Plant Molecular Cytology Laboratory | detail |
Discover the fascinating and untold stories behind the cutting-edge research being conducted by our esteemed professors. Get an in-depth look at the motivations and passions driving their work, and learn how their findings are making a real impact on society. Don't miss the opportunity to gain a deeper understanding of the amazing work being done right here at our laboratory!